How pythons regenerate their organs


EVOLUTION takes aeons, but it leaves marks on the genomes of organisms that can be detected with DNA sequencing and analysis.

As methods for studying and comparing genetic data improve, scientists are beginning to decode these marks to reconstruct the evolutionary history of species, as well as how variants of genes give rise to unique traits.

A research team at the University of Texas at Arlington led by assistant professor of biology Todd Castoe has been exploring the genomes of snakes and lizards to answer critical questions about these creatures’ evolutionary history.

For instance, how did they develop venom? How do they regenerate their organs? And how do evolutionarily- derived variations in genes lead to variations in how organisms look and function?

“Some of the most basic questions drive our research. Yet trying to understand the genetic explanations of such questions is surprisingly difficult considering most vertebrate genomes, including our own, are made up of literally billions of DNA bases that can determine how an organism looks and functions,” says Castoe.

“Understanding these links between differences in DNA and differences in form and function is central to understanding biology and disease, and investigating these critical links requires massive computing power.”

To uncover new insights that link variation in DNA with variation in vertebrate form and function, Castoe’s group uses supercomputing and data analysis resources at the Texas Advanced Computing Center or TACC, one of the world’s leading centres for computational discovery.

Recently, they used TACC’s supercomputers to understand the mechanisms by which Burmese pythons regenerate their organs — including their heart, liver, kidney, and small intestines — after feeding.

Burmese pythons (as well as other snakes) massively downregulate their metabolic and physiological functions during extended periods of fasting. During this time their organs atrophy, saving energy. However, upon feeding, the size and function of these organs, along with their ability to generate energy, dramatically increase to accommodate digestion.

Within 48 hours of feeding, Burmese pythons can undergo up to a 44-fold increase in metabolic rate and the mass of their major organs can increase by 40 to 100 percent. Writing in BMC Genomics in May 2017, the researchers described their efforts to compare gene expression in pythons that were fasting, one-day post-feeding and four days post-feeding. They sequenced pythons in these three states and identified 1,700 genes that were significantly different pre- and post-feeding. They then performed statistical analyses to identify the key drivers of organ regeneration across different types of tissues.

What they found was that a few sets of genes were influencing the wholesale change of pythons’ internal organ structure. Key proteins, produced and regulated by these important genes, activated a cascade of diverse, tissue-specific signals that led to regenerative organ growth.

Intriguingly, even mammalian cells have been shown to respond to serum produced by post-feeding pythons, suggesting that the signalling function is conserved across species and could one day be used to improve human health.

“We’re interested in understanding the molecular basis of this phenomenon to see what genes are regulated related to the feeding response,” says Daren Card, a doctoral student in Castoe’s lab and one of the authors of the study.

“Our hope is that we can leverage our understanding of how snakes accomplish organ regeneration to one day help treat human diseases.”

Castoe and his team used a similar genomic approach to understand gene flow in two closely related species of western rattlesnakes with an intertwined genetic history.

The two species live on opposite sides of the Continental Divide in Mexico and the U.S. They were separated for thousands of years and evolved in response to different climates and habitat.

However, over time their geographic ranges came back together to the point that the rattlesnakes began to crossbreed, leading to hybrids, some of which live in a region between the two distinct climates.

The work was motivated by a desire to understand what forces generate and maintain distinct species, and how shifts in the ranges of species (for example, due to global change) may impact species and speciation.

The researchers compared thousands of genes in the rattlesnakes’ nuclear DNA to study genomic differentiation between the two lineages.

Their comparisons revealed a relationship between genetic traits that are most important in evolution during isolation and those that are most important during secondary contact, with greater-than-expected overlap between genes in these two scenarios.

However, they also found regions of the rattlesnake genome that are important in only one of these two scenarios. For example, genes functioning in venom composition and in reproductive differences — distinct traits that are important for adaptation to the local habitat — likely diverged under selection when these species were isolated.

They also found other sets of genes that were not originally important for diversification of form and function, that later became important in reducing the viability of hybrids. Overall, their results provide a genome-scale perspective on how speciation might work that can be tested and refined in studies of other species. The team published their results in the April 2017 issue of Ecology and Evolution. — University of Texas at Austin